Title | Uniform cell seeding and generation of overlapping gradient profiles in a multiplexed microchamber device with normally-closed valves. |
Publication Type | Journal Article |
Year of Publication | 2010 |
Authors | Mosadegh B, Agarwal M, Tavana H, Bersano-Begey T, Torisawa Y-S, Morell M, Wyatt MJ, K O'Shea S, Barald KF, Takayama S |
Journal | Lab Chip |
Volume | 10 |
Issue | 21 |
Pagination | 2959-64 |
Date Published | 2010 Nov 07 |
ISSN | 1473-0197 |
Keywords | Animals, Embryonic Stem Cells, Mice, Microfluidics |
Abstract | Generation of stable soluble-factor gradients in microfluidic devices enables studies of various cellular events such as chemotaxis and differentiation. However, many gradient devices directly expose cells to constant fluid flow and that can induce undesired responses from cells due to shear stress and/or wash out of cell-secreted molecules. Although there have been devices with flow-free gradients, they typically generate only a single condition and/or have a decaying gradient profile that does not accommodate long-term experiments. Here we describe a microdevice that generates several chemical gradient conditions on a single platform in flow-free microchambers which facilitates steady-state gradient profiles. The device contains embedded normally-closed valves that enable fast and uniform seeding of cells to all microchambers simultaneously. A network of microchannels distributes desired solutions from easy-access open reservoirs to a single output port, enabling a simple setup for inducing flow in the device. Embedded porous filters, sandwiched between the microchannel networks and cell microchambers, enable diffusion of biomolecules but inhibit any bulk flow over the cells. |
DOI | 10.1039/c0lc00086h |
Alternate Journal | Lab Chip |
PubMed ID | 20835429 |
Grant List | HL-084370 / HL / NHLBI NIH HHS / United States |
Related Institute:
Dalio Institute of Cardiovascular Imaging (Dalio ICI)