Transposition into replicating DNA occurs through interaction with the processivity factor.

TitleTransposition into replicating DNA occurs through interaction with the processivity factor.
Publication TypeJournal Article
Year of Publication2009
AuthorsParks AR, Li Z, Shi Q, Owens RM, Jin MM, Peters JE
JournalCell
Volume138
Issue4
Pagination685-95
Date Published2009 Aug 21
ISSN1097-4172
KeywordsAmino Acid Sequence, DNA Polymerase III, DNA Replication, DNA Transposable Elements, DNA-Binding Proteins, Escherichia coli, Escherichia coli Proteins, Molecular Sequence Data, Sequence Alignment
Abstract

The bacterial transposon Tn7 directs transposition into actively replicating DNA by a mechanism involving the transposon-encoded protein TnsE. Here we show that TnsE physically and functionally interacts with the processivity factor of the DNA replication machinery in vivo and in vitro. Our work establishes an in vitro TnsABC+E transposition reaction reconstituted from purified proteins and target DNA structures. Using the in vitro reaction we confirm that the processivity factor specifically reorders TnsE-mediated transposition events on target DNAs in a way that matches the bias with active DNA replication in vivo. The TnsE interaction with an essential and conserved component of the replication machinery, and a DNA structure reveals a mechanism by which Tn7, and probably other elements, selects target sites associated with DNA replication.

DOI10.1016/j.cell.2009.06.011
Alternate JournalCell
PubMed ID19703395
PubMed Central IDPMC2752310
Grant ListR01 GM069508 / GM / NIGMS NIH HHS / United States
R01 GM069508-03 / GM / NIGMS NIH HHS / United States
Related Institute: 
Molecular Imaging Innovations Institute (MI3)

Weill Cornell Medicine
Department of Radiology
525 East 68th Street New York, NY 10065