| Title | siRNA Nanoparticles for Ultra-Long Gene Silencing In Vivo. |
| Publication Type | Journal Article |
| Year of Publication | 2016 |
| Authors | Lee SKoo, Tung C-H |
| Journal | Methods Mol Biol |
| Volume | 1372 |
| Pagination | 113-20 |
| Date Published | 2016 |
| ISSN | 1940-6029 |
| Keywords | Animals, Cell Line, Tumor, Gene Expression, Gene Silencing, Gold, Heterografts, Humans, Metal Nanoparticles, Mice, Models, Animal, Molecular Imaging, Nanoparticles, RNA Interference, RNA, Small Interfering |
| Abstract | Small interfering RNA (siRNA)-mediated gene silencing has shown prominent therapeutic effects in treating various diseases. However, adequate delivery and persistent gene silencing remain challenging. A nanoparticle-based delivery system which assembled by layering siRNAs between protease degradable polypeptides to show ultra-long gene silencing effect in vivo is developed. Gold nanoparticle is used as a scaffold for its unique properties including uniform size, biocompatibility, ready synthesis, and easy functionalization. A simple layer-by-layer fabrication approach, based on the electrostatic interaction between positively and negatively charged polymers, is applied to package the therapeutic siRNAs. |
| DOI | 10.1007/978-1-4939-3148-4_9 |
| Alternate Journal | Methods Mol Biol |
| PubMed ID | 26530919 |
| Grant List | CA135312 / CA / NCI NIH HHS / United States |
Related Institute:
Molecular Imaging Innovations Institute (MI3)