| Title | Resolution enhanced NMR spectroscopy in biological systems via magnetic susceptibility matched sample immersion chambers. |
| Publication Type | Journal Article |
| Year of Publication | 1993 |
| Authors | Balloon D, Mahmood U, Jakubowski A, Koutcher JA |
| Journal | Magn Reson Med |
| Volume | 30 |
| Issue | 6 |
| Pagination | 754-8 |
| Date Published | 1993 Dec |
| ISSN | 0740-3194 |
| Keywords | Animals, Fibrosarcoma, Leukemia, Experimental, Magnetic Resonance Spectroscopy, Mammary Neoplasms, Experimental, Mice, Mice, Inbred C3H, Tumor Cells, Cultured |
| Abstract | A technique is described which reduced the magnetic susceptibility induced line broadening in NMR spectra obtained from three biological systems at 4.7 Tesla. Proton spectra from a sealed suspension of HL60 leukemic myeloblasts yielded minimum linewidths of 1.3 Hz at 200 MHz (0.0065 ppm) after 10 min of automated shimming. 31P spectra from an in vivo murine MCa mammary carcinoma yielded a well-resolved phosphorylcholine resonance without proton decoupling and with the resistive shim coil currents set to zero. 31P spectra from a perfused suspension of RIF-1 fibrosarcoma cells exhibited a gamma-nucleoside triphosphate resonance which was resolved into purine and pyrimidine components. |
| DOI | 10.1002/mrm.1910300615 |
| Alternate Journal | Magn Reson Med |
| PubMed ID | 8139459 |
| Grant List | CA 25842 / CA / NCI NIH HHS / United States CM-87215-18 / CM / NCI NIH HHS / United States |
Related Institute:
MRI Research Institute (MRIRI)