Reproducibility of single- and multi-voxel 1H MRS measurements of intramyocellular lipid in overweight and lean subjects under conditions of controlled dietary calorie and fat intake.

TitleReproducibility of single- and multi-voxel 1H MRS measurements of intramyocellular lipid in overweight and lean subjects under conditions of controlled dietary calorie and fat intake.
Publication TypeJournal Article
Year of Publication2008
AuthorsShen W, Mao X, Wolper C, Heshka S, Dashnaw S, Hirsch J, Heymsfield SB, Shungu DC
JournalNMR Biomed
Volume21
Issue5
Pagination498-506
Date Published2008 Jun
ISSN0952-3480
KeywordsAdult, Biomedical Research, Caloric Restriction, Creatinine, Diet, Fat-Restricted, Female, Humans, Hydrogen, Leg, Lipid Metabolism, Lipids, Magnetic Resonance Imaging, Magnetic Resonance Spectroscopy, Muscle Fibers, Skeletal, Overweight, Reference Values, Reproducibility of Results, Research Design
Abstract

The reproducibility of repeated single-voxel 1H MRS (SV-MRS) and spectroscopic imaging (MRSI) measurements of intramyocellular lipid (IMCL) in the tibialis anterior muscle of five lean and five overweight female Caucasians, during 7 days of controlled dietary fat and calorie intake, was assessed at 1.5 T. Duplicate measures of IMCL relative to total muscle creatine (IMCL/tCr) obtained 3 days apart by both SV-MRS and MRSI correlated well (r = 0.65 and r = 0.95, respectively, P < 0.05). The coefficients of variation for repeated measures of IMCL/tCr by SV-MRS and MRSI were 24.4% and 10.7%, respectively. IMCL/tCr measured by MRSI was higher in overweight subjects than in lean subjects (8.3 +/- 3.8 vs 4.3 +/- 2.4, P < 0.05). Although both methods achieved good reproducibility in measuring IMCL in vivo, MRSI was found to offer greater flexibility and reliability, and higher sensitivity to IMCL differences, whereas SV-MRS was advantageous with respect to shorter scan time and ease of implementation.

DOI10.1002/nbm.1218
Alternate JournalNMR Biomed
PubMed ID17955571
PubMed Central IDPMC2892914
Grant ListP30 DK026687 / DK / NIDDK NIH HHS / United States
P30 DK026687-26 / DK / NIDDK NIH HHS / United States
P30 DK026687-279012 / DK / NIDDK NIH HHS / United States
Related Institute: 
MRI Research Institute (MRIRI)

Weill Cornell Medicine
Department of Radiology
525 East 68th Street New York, NY 10065