| Title | Protease-sensitive fluorescent nanofibers. |
| Publication Type | Journal Article |
| Year of Publication | 2007 |
| Authors | Law B, Weissleder R, Tung C-H |
| Journal | Bioconjug Chem |
| Volume | 18 |
| Issue | 6 |
| Pagination | 1701-4 |
| Date Published | 2007 Nov-Dec |
| ISSN | 1043-1802 |
| Keywords | Fluorescence, Microscopy, Electron, Transmission, Nanostructures, Sensitivity and Specificity, Spectrometry, Fluorescence, Substrate Specificity, Urokinase-Type Plasminogen Activator |
| Abstract | We report the design and synthesis of enzyme-responsive nanofibers. The fibers are composed of self-assembled hydrophobic beta-sheet peptides incorporating protease-sensitive domains, fluorescent reporters, and hydrophilic poly(ethylene glycol) (PEG) units. Using urokinase plasminogen activator (uPA) as a model system, nanofibers were developed to release fluorescent fragments upon uPA incubation. These protease-sensitive nanofibers may have considerable biomedical applications as diagnostic sensors or for protease-assisted drug deliveries. |
| DOI | 10.1021/bc070054z |
| Alternate Journal | Bioconjug Chem |
| PubMed ID | 17915958 |
| PubMed Central ID | PMC2528070 |
| Grant List | R33 CA114149 / CA / NCI NIH HHS / United States P50 CA086355 / CA / NCI NIH HHS / United States R33 CA114149-03 / CA / NCI NIH HHS / United States P50 CA86355 / CA / NCI NIH HHS / United States R33 CA114149-02 / CA / NCI NIH HHS / United States R01 CA099385 / CA / NCI NIH HHS / United States R01 CA099385-04 / CA / NCI NIH HHS / United States |
Related Institute:
Molecular Imaging Innovations Institute (MI3)