Title | Microprinted feeder cells guide embryonic stem cell fate. |
Publication Type | Journal Article |
Year of Publication | 2011 |
Authors | Tavana H, Mosadegh B, Zamankhan P, Grotberg JB, Takayama S |
Journal | Biotechnol Bioeng |
Volume | 108 |
Issue | 10 |
Pagination | 2509-16 |
Date Published | 2011 Oct |
ISSN | 1097-0290 |
Keywords | Animals, Cell Differentiation, Cell Line, Coculture Techniques, Embryonic Stem Cells, Feeder Cells, Mice, Microarray Analysis, Neurons |
Abstract | We introduce a non-contact approach to microprint multiple types of feeder cells in a microarray format using immiscible aqueous solutions of two biopolymers. Droplets of cell suspension in the denser aqueous phase are printed on a substrate residing within a bath of the immersion aqueous phase. Due to their affinity to the denser phase, cells remain localized within the drops and adhere to regions of the substrate underneath the drops. We show the utility of this technology for creating duplex heterocellular stem cell niches by printing two different support cell types on a gel surface and overlaying them with mouse embryonic stem cells (mESCs). As desired, the type of printed support cell spatially direct the fate of overlaid mESCs. Interestingly, we found that interspaced mESCs colonies on differentiation-inducing feeder cells show enhanced neuronal differentiation and give rise to dense networks of neurons. This cell printing technology provides unprecedented capabilities to efficiently identify the role of various feeder cells in guiding the fate of stem cells. |
DOI | 10.1002/bit.23190 |
Alternate Journal | Biotechnol Bioeng |
PubMed ID | 21538333 |
PubMed Central ID | PMC3183395 |
Grant List | R01 HL084370 / HL / NHLBI NIH HHS / United States R01 HL084370-05 / HL / NHLBI NIH HHS / United States HL084370 / HL / NHLBI NIH HHS / United States |
Related Institute:
Dalio Institute of Cardiovascular Imaging (Dalio ICI)