Title | Micro-ring structures stabilize microdroplets to enable long term spheroid culture in 384 hanging drop array plates. |
Publication Type | Journal Article |
Year of Publication | 2012 |
Authors | Hsiao AY, Tung Y-C, Kuo C-H, Mosadegh B, Bedenis R, Pienta KJ, Takayama S |
Journal | Biomed Microdevices |
Volume | 14 |
Issue | 2 |
Pagination | 313-23 |
Date Published | 2012 Apr |
ISSN | 1572-8781 |
Keywords | Cell Culture Techniques, Cell Line, Tumor, Equipment Design, High-Throughput Screening Assays, Humans, Spheroids, Cellular |
Abstract | Using stereolithography, 20 different structural variations comprised of millimeter diameter holes surrounded by trenches, plateaus, or micro-ring structures were prepared and tested for their ability to stably hold arrays of microliter sized droplets within the structures over an extended period of time. The micro-ring structures were the most effective in stabilizing droplets against mechanical and chemical perturbations. After confirming the importance of micro-ring structures using rapid prototyping, we developed an injection molding tool for mass production of polystyrene 3D cell culture plates with an array of 384 such micro-ring surrounded through-hole structures. These newly designed and injection molded polystyrene 384 hanging drop array plates with micro-rings were stable and robust against mechanical perturbations as well as surface fouling-facilitated droplet spreading making them capable of long term cell spheroid culture of up to 22 days within the droplet array. This is a significant improvement over previously reported 384 hanging drop array plates which are susceptible to small mechanical shocks and could not reliably maintain hanging drops for longer than a few days. With enhanced droplet stability, the hanging drop array plates with micro-ring structures provide better platforms and open up new opportunities for high-throughput preparation of microscale 3D cell constructs for drug screening and cell analysis. |
DOI | 10.1007/s10544-011-9608-5 |
Alternate Journal | Biomed Microdevices |
PubMed ID | 22057945 |
PubMed Central ID | PMC3304008 |
Grant List | P50 CA069568 / CA / NCI NIH HHS / United States P50 CA069568-15 / CA / NCI NIH HHS / United States |
Related Institute:
Dalio Institute of Cardiovascular Imaging (Dalio ICI)