| Title | Macrocyclic chelators with paramagnetic cations are internalized into mammalian cells via a HIV-tat derived membrane translocation peptide. |
| Publication Type | Journal Article |
| Year of Publication | 2000 |
| Authors | Bhorade R, Weissleder R, Nakakoshi T, Moore A, Tung CH |
| Journal | Bioconjug Chem |
| Volume | 11 |
| Issue | 3 |
| Pagination | 301-5 |
| Date Published | 2000 May-Jun |
| ISSN | 1043-1802 |
| Keywords | Amino Acid Sequence, Animals, Biological Transport, Cell Membrane, Cell Nucleus, Chelating Agents, Cytoplasm, Dysprosium, Gadolinium, Gene Products, tat, HeLa Cells, Heterocyclic Compounds, 1-Ring, Humans, Indium Radioisotopes, Lymphocytes, Magnetic Resonance Imaging, Mice, Molecular Sequence Data, Peptide Fragments, tat Gene Products, Human Immunodeficiency Virus |
| Abstract | A major obstacle to using paramagnetic MR contrast agents for in vivo cell tracking or molecular sensing is their generally low cellular uptake. In this study, we show that a paramagnetically labeled DOTA chelator derivatized with a 13-mer HIV-tat peptide is efficiently internalized into mammalian cells. Intracellular concentrations were attained that were readily detectable by MR imaging using both gadolinium and dysprosium chelates. Using this paradigm, it should be feasible to internalize a variety of chemically different agents into mammalian cells. |
| DOI | 10.1021/bc990168d |
| Alternate Journal | Bioconjug Chem |
| PubMed ID | 10821645 |
| Grant List | R01AI-CA46973 / AI / NIAID NIH HHS / United States R21DK55713 / DK / NIDDK NIH HHS / United States |
Related Institute:
Molecular Imaging Innovations Institute (MI3)