| Title | A fluorogenic probe for β-galactosidase activity imaging in living cells. |
| Publication Type | Journal Article |
| Year of Publication | 2013 |
| Authors | Han J, Han MShin, Tung C-H |
| Journal | Mol Biosyst |
| Volume | 9 |
| Issue | 12 |
| Pagination | 3001-8 |
| Date Published | 2013 Dec |
| ISSN | 1742-2051 |
| Keywords | beta-Galactosidase, Carbocyanines, Cell Line, Tumor, Culture Media, Cytological Techniques, Escherichia coli Proteins, Fluorescent Dyes, Humans, Indoles, Microscopy, Fluorescence |
| Abstract | A cell permeable fluorescence turn-on probe, AcGQCy7, was developed to image β-galactosidase activity in living cells. Once internalized by β-galactosidase-expressing cells, the probe was hydrolyzed into a highly fluorescent molecule, and the fluorescent signal was retained in mitochondria for several days. This resulted in a long-lasting and strong β-galactosidase-dependent intracellular fluorescent signal with little background fluorescence in the culture media. |
| DOI | 10.1039/c3mb70269c |
| Alternate Journal | Mol Biosyst |
| PubMed ID | 24056749 |
| PubMed Central ID | PMC3836597 |
| Grant List | R01 CA135312 / CA / NCI NIH HHS / United States P30 CA016672 / CA / NCI NIH HHS / United States CA135312 / CA / NCI NIH HHS / United States GM 094880 / GM / NIGMS NIH HHS / United States R01 GM094880 / GM / NIGMS NIH HHS / United States |
Related Institute:
Molecular Imaging Innovations Institute (MI3)