| Title | A fluorescent nanosensor for apoptotic cells. |
| Publication Type | Journal Article |
| Year of Publication | 2006 |
| Authors | Quinti L, Weissleder R, Tung C-H |
| Journal | Nano Lett |
| Volume | 6 |
| Issue | 3 |
| Pagination | 488-90 |
| Date Published | 2006 Mar |
| ISSN | 1530-6984 |
| Keywords | Apoptosis, Biosensing Techniques, Flow Cytometry, Fluorescence, Fluorescent Dyes, Humans, Jurkat Cells, Molecular Structure, Nanostructures, Nanotechnology |
| Abstract | A biocompatible surface-functionalized nanoparticle was designed to sense phosphatidylserine exposed on apoptotic cells. We conjugated synthetic artificial phosphatidylserine binding ligands in a multivalent fashion onto magnetofluorescent nanoparticles. Our results show that (1) the synthetic nanoparticles bind to apoptotic cells, (2) there is excellent correlation with annexin V staining by microscopy, and (3) FACS analysis with nanoparticles allows the measurement of therapeutic apoptosis induction. The described nanomaterials should be useful for a variety of biomedical applications including in vivo imaging of apoptosis. |
| DOI | 10.1021/nl0524694 |
| Alternate Journal | Nano Lett |
| PubMed ID | 16522048 |
| Grant List | P50-CA86355 / CA / NCI NIH HHS / United States R01 CA99385 / CA / NCI NIH HHS / United States R21 CA114149 / CA / NCI NIH HHS / United States |
Related Institute:
Molecular Imaging Innovations Institute (MI3)