A fabricated siRNA nanoparticle for ultra-long gene silencing

TitleA fabricated siRNA nanoparticle for ultra-long gene silencing
Publication TypeJournal Article
Year of Publication2013
AuthorsLee SKoo, Tung C-H
JournalAdv Funct Mater
Volume23
Issue28
Pagination3488-3493
Date Published2013 07 26
ISSN1616-301X
Abstract

Persistent gene silencing is crucially required for the successful therapeutics of short interfering RNA (siRNA). Here, we describe a nanoparticle based delivery system which assembled by layering siRNAs between protease degradable polypeptides to extend the therapeutic window. These tightly packed nanoparticles are efficiently taken up by cells by endocytosis, and the fabricated siRNAs are gradually released following intracellular degradation of the polypeptide layers. During cell division, the particles are distributed to the daughter cells. Due to the slow degradation through the multiple layers, the particles continuously release siRNA in all cells. Using this controlled release construct, the gene silencing effect of siRNA is consistent for an ultra-long period of time (>3 weeks) with only a single treatment.

DOI10.1002/adfm.201202777
Alternate JournalAdv Funct Mater
PubMed ID24999314
PubMed Central IDPMC4078887
Grant ListR01 CA135312 / CA / NCI NIH HHS / United States
Related Institute: 
Molecular Imaging Innovations Institute (MI3)

Weill Cornell Medicine
Department of Radiology
525 East 68th Street New York, NY 10065