| Title | Equilibrium binding constants for the group I metal cations with gramicidin-A determined by competition studies and T1+-205 nuclear magnetic resonance spectroscopy. |
| Publication Type | Journal Article |
| Year of Publication | 1986 |
| Authors | Hinton JF, Whaley WL, Shungu D, Koeppe RE, Millett FS |
| Journal | Biophys J |
| Volume | 50 |
| Issue | 3 |
| Pagination | 539-44 |
| Date Published | 1986 Sep |
| ISSN | 0006-3495 |
| Keywords | Cations, Monovalent, Gramicidin, Kinetics, Lysophosphatidylcholines, Magnetic Resonance Spectroscopy, Models, Biological, Models, Molecular, Protein Binding, Thallium |
| Abstract | The equilibrium binding constants of the Group I metal cations with gramicidin A in aqueous dispersions of lyso-PC have been determined using a combination of competitive binding with the T1+ ion and T1-205 NMR spectroscopy. The values of the binding constants at 34 degrees C are Li (32.2 M-1), Na (36.9 M-1), K (52.6 M-1), Rb (55.9 M-1), and Cs (54.0 M-1). The equilibrium binding constant for the T1+ ion at this temperature is 582 M-1. The relationships between the binding constants, the free energy of the binding process, and the cation selectivity of the gramicidin A channel are discussed. |
| DOI | 10.1016/S0006-3495(86)83492-0 |
| Alternate Journal | Biophys J |
| PubMed ID | 2428415 |
| PubMed Central ID | PMC1329731 |
| Grant List | GM-34968 / GM / NIGMS NIH HHS / United States NS-00648 / NS / NINDS NIH HHS / United States |
Related Institute:
MRI Research Institute (MRIRI)