Antioxidant status and energy state of erythrocytes in Alzheimer dementia: probing for markers.

TitleAntioxidant status and energy state of erythrocytes in Alzheimer dementia: probing for markers.
Publication TypeJournal Article
Year of Publication2012
AuthorsKosenko EA, Aliev G, Tikhonova LA, Li Y, Poghosyan AC, Kaminsky YG
JournalCNS Neurol Disord Drug Targets
Volume11
Issue7
Pagination926-32
Date Published2012 Nov 01
ISSN1996-3181
KeywordsAdenosine Triphosphate, Adult, Aged, Aged, 80 and over, Aging, Alzheimer Disease, Biomarkers, Dementia, Energy Metabolism, Erythrocytes, Glutathione, Glutathione Peroxidase, Glutathione Transferase, Humans, Male, Middle Aged, Oxidation-Reduction, Oxidative Stress, Oxidoreductases, Peroxides
Abstract

Subject age and brain oxidative stress play pivotal roles in Alzheimer disease (AD) pathology. Erythrocytes (red blood cells: RBC) are considered as passive "reporter cells" for the oxidative status of the whole organism, not active participants in mechanisms of AD pathogenesis and are not well studied in AD. The aim of this work is to assess whether the antioxidant status and energy state of RBC from elderly people change in AD. We measured levels of key products and enzymes of oxidative metabolism in RBC from AD (n = 12) and non-Alzheimer dementia (NA, n = 13) patients, as well as in cells from age-matched controls (AC, n = 14) and younger adult controls (YC, n = 14). Parameters of the adenylate system served to evaluate the energy state of RBC. In both aging and dementia, oxidative stress in RBC increased and exhibited elevated concentrations of H₂O₂ and organic hydroperoxides, decreased the GSH/GSSG ratio and glutathione-S-transferase activity. Reductions in the ATP levels, adenine nucleotide pool size (AN) and adenylate energy charge accompanied these oxidative disturbances. The patterns of changes in these indices between groups strongly correlated with each other, Spearman rank correlation coefficients being r(s) = 1.0 or -1.0 (p < 0.01). Alterations of the RBC parameters of oxidative stress and adenylate metabolism were nonspecific and interpreted as age-related abnormalities. Decreased glutathione peroxidase activity in RBC may be considered as a new peripheral marker for AD.

DOI10.2174/1871527311201070926
Alternate JournalCNS Neurol Disord Drug Targets
PubMed ID22998137
Related Institute: 
Brain Health Imaging Institute (BHII)

Weill Cornell Medicine
Department of Radiology
525 East 68th Street New York, NY 10065