| Title | Oligonucleotide-poly-L-ornithine conjugates: binding to complementary DNA and RNA. |
| Publication Type | Journal Article |
| Year of Publication | 1993 |
| Authors | Zhu T, Wei Z, Tung CH, Dickerhof WA, Breslauer KJ, Georgopoulos DE, Leibowitz MJ, Stein S |
| Journal | Antisense Res Dev |
| Volume | 3 |
| Issue | 3 |
| Pagination | 265-75 |
| Date Published | 1993 Fall |
| ISSN | 1050-5261 |
| Keywords | Base Sequence, DNA, Complementary, Molecular Sequence Data, Mutagenesis, Site-Directed, Nucleic Acid Hybridization, Oligodeoxyribonucleotides, Oligonucleotides, Antisense, Peptides, Polylysine, Protein Biosynthesis, Ribonuclease H, RNA, Complementary, Transcription, Genetic |
| Abstract | On the basis of the reported enhanced antisense activity of polylysine-oligonucleotide conjugates, a synthetic 12-mer oligodeoxyribonucleotide has been coupled at its 5' terminus to a series of positively charged (delta-ornithine)n cysteine peptides. Binding between the nucleic acid-peptide conjugate and its complementary DNA target sequence was detected by the impact of complexation on the melting temperature (Tm). It was found that the Tm for the nucleic acid-peptide gradually increased with increasing net charge on the conjugated peptide. Site-directed cleavage with RNase H demonstrates that the peptide-modified oligomer also hybridizes with its RNA target sequence. Increased affinity for target mRNA with net charge was shown by a cell-free translation arrest assay. |
| DOI | 10.1089/ard.1993.3.265 |
| Alternate Journal | Antisense Res Dev |
| PubMed ID | 8286927 |
Related Institute:
Molecular Imaging Innovations Institute (MI3)