Dual-specificity interaction of HIV-1 TAR RNA with Tat peptide-oligonucleotide conjugates.

TitleDual-specificity interaction of HIV-1 TAR RNA with Tat peptide-oligonucleotide conjugates.
Publication TypeJournal Article
Year of Publication1995
AuthorsTung CH, Wang J, Leibowitz MJ, Stein S
JournalBioconjug Chem
Volume6
Issue3
Pagination292-5
Date Published1995 May-Jun
ISSN1043-1802
KeywordsAmino Acid Sequence, Base Sequence, Binding Sites, Gene Products, tat, HIV Long Terminal Repeat, HIV-1, Molecular Sequence Data, Nucleic Acid Conformation, Oligonucleotides, Peptides, RNA, Viral, tat Gene Products, Human Immunodeficiency Virus
Abstract

An oligonucleotide-peptide conjugate, having dual binding capability for a designated RNA, was designed. The peptide portion of the conjugate interacts with a folded domain in the RNA, whereas the oligonucleotide portion hybridizes with a nearby single-stranded region in the RNA. The dual specificity was proven in a model HIV-1 TAR RNA system using an RNase H cleavage assay to assess antisense binding to this RNA. The peptide portion of the conjugate was shown to confer increased specificity on the oligonucleotide.

DOI10.1021/bc00033a009
Alternate JournalBioconjug Chem
PubMed ID7632801
Related Institute: 
Molecular Imaging Innovations Institute (MI3)

Weill Cornell Medicine
Department of Radiology
525 East 68th Street New York, NY 10065