| Title | Dual-specificity interaction of HIV-1 TAR RNA with Tat peptide-oligonucleotide conjugates. |
| Publication Type | Journal Article |
| Year of Publication | 1995 |
| Authors | Tung CH, Wang J, Leibowitz MJ, Stein S |
| Journal | Bioconjug Chem |
| Volume | 6 |
| Issue | 3 |
| Pagination | 292-5 |
| Date Published | 1995 May-Jun |
| ISSN | 1043-1802 |
| Keywords | Amino Acid Sequence, Base Sequence, Binding Sites, Gene Products, tat, HIV Long Terminal Repeat, HIV-1, Molecular Sequence Data, Nucleic Acid Conformation, Oligonucleotides, Peptides, RNA, Viral, tat Gene Products, Human Immunodeficiency Virus |
| Abstract | An oligonucleotide-peptide conjugate, having dual binding capability for a designated RNA, was designed. The peptide portion of the conjugate interacts with a folded domain in the RNA, whereas the oligonucleotide portion hybridizes with a nearby single-stranded region in the RNA. The dual specificity was proven in a model HIV-1 TAR RNA system using an RNase H cleavage assay to assess antisense binding to this RNA. The peptide portion of the conjugate was shown to confer increased specificity on the oligonucleotide. |
| DOI | 10.1021/bc00033a009 |
| Alternate Journal | Bioconjug Chem |
| PubMed ID | 7632801 |
Related Institute:
Molecular Imaging Innovations Institute (MI3)