Title | Butyrate response factor 1 enhances cisplatin sensitivity in human head and neck squamous cell carcinoma cell lines. |
Publication Type | Journal Article |
Year of Publication | 2005 |
Authors | Lee SKoo, Kim SBum, Kim JSoo, Moon CHoon, Han MShin, Lee BJu, Chung DKyun, Min YJoo, Park JHoo, Choi DHwa, Cho HRae, Park SKyu, Park JWoo |
Journal | Int J Cancer |
Volume | 117 |
Issue | 1 |
Pagination | 32-40 |
Date Published | 2005 Oct 20 |
ISSN | 0020-7136 |
Keywords | Antineoplastic Agents, Apoptosis, Base Sequence, Biomarkers, Tumor, Butyrate Response Factor 1, Carcinoma, Squamous Cell, Caspase 3, Caspases, Cisplatin, DNA-Binding Proteins, Drug Resistance, Neoplasm, Gene Expression Profiling, Head and Neck Neoplasms, Humans, Immediate-Early Proteins, Molecular Sequence Data, Oligonucleotide Array Sequence Analysis, Proteins, Tumor Cells, Cultured |
Abstract | Cisplatin is a widely used chemotherapeutic agent in head and neck squamous cell carcinoma (HNSCC). Resistance to cisplatin is a common feature of HNSCC. To identify genes that may regulate cisplatin sensitivity, we carried out a cDNA microarray analysis of gene expression in cisplatin-sensitive and cisplatin-resistant HNSCC-derived cell lines. Among genes differentially expressed by cisplatin treatment, we have confirmed the elevated expression of butyrate responsive factor 1 (BRF1) in cisplatin-sensitive HNSCC cells and have demonstrated that the expression level of BRF1 is associated with cisplatin-sensitivity. Specific inhibition of BRF1 expression using an antisense oligodeoxynucleotide (ODN) decreased the cisplatin-sensitivity and, on the contrary, overexpression of BRF1 increased cisplatin-sensitivity in HNSCC cells. Elevated expression of BRF1 decreased the level of the human inhibitor of apoptosis protein-2 (cIAP2) and increased the caspase-3 activity in HNSCC cells. In addition, elevated expression of BRF1 decreased the expression level of enhanced green fluorescent protein (EGFP) linked to a 3' terminal AU-rich element (ARE) of cIAP2 mRNA. These findings demonstrate that BRF1 expression enhanced cisplatin sensitivity in HNSCC cells by reducing the levels of cIAP2 mRNA. |
DOI | 10.1002/ijc.21133 |
Alternate Journal | Int J Cancer |
PubMed ID | 15880358 |
Related Institute:
Molecular Imaging Innovations Institute (MI3)