Title | Lipo-oligoarginine-based intracellular delivery. |
Publication Type | Journal Article |
Year of Publication | 2013 |
Authors | Lee JSam, Tung C-H |
Journal | Methods Mol Biol |
Volume | 991 |
Pagination | 281-92 |
Date Published | 2013 |
ISSN | 1940-6029 |
Keywords | Arginine, Drug Delivery Systems, Humans, Jurkat Cells, Microscopy, Confocal, Microscopy, Fluorescence |
Abstract | Efficient cellular delivery, including plasma membrane permeability and intracellular metabolic stability, is a crucial factor determining the success of therapeutic agents. Cell-penetrating peptides (CPPs) have been widely used for the intracellular delivery of various bioactive molecules into cells to modify cellular functions. We have developed an improved CPP-based cellular delivery vector, named lipo-oligoarginine peptide (LOAP), by conjugating an oligoarginine peptide with a fatty acid moiety. The prepared LOAPs were further stabilized by introducing different combinations of D-Arg residues into the peptide backbone and were systematically evaluated for their membrane-penetrating properties and metabolic stabilities in cells. |
DOI | 10.1007/978-1-62703-336-7_26 |
Alternate Journal | Methods Mol Biol |
PubMed ID | 23546678 |
Grant List | CA114149 / CA / NCI NIH HHS / United States CA126752 / CA / NCI NIH HHS / United States CA135312 / CA / NCI NIH HHS / United States |
Related Institute:
Molecular Imaging Innovations Institute (MI3)